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Context: Stalk lodging causes up to 43 % of yield losses in maize (Zea mays L.) worldwide, significantly worsening food and feed shortages. Stalk lodging resistance is a complex trait specified by several structural, material, and geometric phenotypes. However, the identity, relative contribution, and genetic tractability of these intermediate phenotypes remain unknown. Objective: The study is designed to identify and evaluate plant-, organ-, and tissue-level intermediate phenotypes associated with stalk lodging resistance following standardized phenotyping protocols and to understand the variation and genetic tractability of these intermediate phenotypes. Methods: We examined 16 diverse maize hybrids in two environments to identify and evaluate intermediate phenotypes associated with stalk flexural stiffness, a reliable indicator of stalk lodging resistance, at physiological maturity. Engineering-informed and machine learning models were employed to understand relationships among intermediate phenotypes and stalk flexural stiffness. Results: Stalk flexural stiffness showed significant genetic variation and high heritability (0.64) in the evaluated hybrids. Significant genetic variation and comparable heritability for the cross-sectional moment of inertia and Young’s modulus indicated that geometric and material properties are under tight genetic control and play a combinatorial role in determining stalk lodging resistance. Among the twelve internode-level traits measured on the bottom and the ear internode, most traits exhibited significant genetic variation among hybrids, moderate to high heritability, and considerable effect of genotype × environment interaction. The marginal statistical model based on structural engineering beam theory revealed that 74–80 % of the phenotypic variation for flexural stiffness was explained by accounting for the major diameter, minor diameter, and rind thickness of the stalks. The machine learning model explained a relatively modest proportion (58–62 %) of the variation for flexural stiffness. 

e. With recent advances in online sensing technology and high-performance computing, structural health monitoring (SHM) has begun to emerge as an automated approach to the real-time conditional monitoring of civil infrastructure. Ideal SHM strategies detect and characterize damage by leveraging measured response data to update physics-based finite element models (FEMs). When monitoring composite structures, such as reinforced concrete (RC) bridges, the reliability of FEM based SHM is adversely affected by material, boundary, geometric, and other model uncertainties. Civil engineering researchers have adapted popular artificial intelligence (AI) techniques to overcome these limitations, as AI has an innate ability to solve complex and ill-defined problems by leveraging advanced machine learning techniques to rapidly analyze experimental data. In this vein, this study employs a novel Bayesian estimation technique to update a coupled vehicle-bridge FEM for the purposes of SHM. Unlike existing AI based techniques, the proposed approach makes intelligent use of an embedded FEM model, thus reducing the parameter space while simultaneously guiding the Bayesian model via physics-based principles. To validate the method, bridge response data is generated from the vehicle-bridge FEM given a set of “true” parameters and the bias and standard deviation of the parameter estimates are analyzed. Additionally, the mean parameter estimates are used to solve the FEM model and the results are compared against the results obtained for “true” parameter values. A sensitivity study is also conducted to demonstrate methods for properly formulating model spaces to improve the Bayesian estimation routine. The study concludes with a discussion highlighting factors that need to be considered when leveraging experimental data to update FEMs of concrete structures using AI techniques. 

Summary In screening applications involving low-prevalence diseases, pooling specimens (e.g., urine, blood, swabs, etc.) through group testing can be far more cost effective than testing specimens individually. Estimation is a common goal in such applications and typically involves modeling the probability of disease as a function of available covariates. In recent years, several authors have developed regression methods to accommodate the complex structure of group testing data but often under the assumption that covariate effects are linear. Although linearity is a reasonable assumption in some applications, it can lead to model misspecification and biased inference in others. To offer a more flexible framework, we propose a Bayesian generalized additive regression approach to model the individual-level probability of disease with potentially misclassified group testing data. Our approach can be used to analyze data arising from any group testing protocol with the goal of estimating multiple unknown smooth functions of covariates, standard linear effects for other covariates, and assay classification accuracy probabilities. We illustrate the methods in this article using group testing data on chlamydia infection in Iowa.